Cas proteins (CRISPR-associated proteins) are DNA or RNA-binding proteins near CRISPR sequences that also have nuclease functions. These proteins enable programmable genome editing and epigenetic regulation, with applications in homologous-directed repair, gene knockout, chromosomal translocation, single-base editing, chromatin remodeling, and clinical research.

Despite the powerful capabilities of Cas proteins in gene editing, there are limitations. Different Cas proteins vary in cleavage efficiency and specificity, affecting editing accuracy and efficiency. The delivery efficiency of the CRISPR-Cas system in vivo is relatively low, necessitating the development of more efficient vector systems. Off-target effects and immune response issues also need to be addressed to ensure safety and efficacy in clinical treatments. In this regard, high-quality, high-purity Cas enzyme production and customization services become essential.