dCas9 can be fused with a gene repressor protein, the KRAB protein, to form a complex CRISPRi (CRISPR interference) that inhibits gene expression. dCas9-KRAB targeting to the promoter region causes steric hindrance that halts transcript elongation by RNA polymerase, resulting in the target gene being repressed. CRISPR-based gene interference provides an efficient and specific genome-targeting platform for transcriptional control without altering the target DNA sequence.

CRISPR/Cas9 PlatformCB provides a complete and powerful solution for CRISPR gene interference helping you to specifically suppress the expression of any gene. Our custom CRISPRi sgRNA vectors can be used on a variety of cells, including easy-to-transfect and difficult-to-transfect cells, covering the entire genome of humans, mice, and rats. Just send us the gene ID you are interested in, we will design, clone and provide this powerful technology for you.

The CRISPR/Cas9 PlatformCB is a professional company dedicated to developing and optimizing CRISPR system application solutions for basic research, human disease research and drug discovery. Our work is based on a thorough understanding of the CRISPR system and years of experience in gene editing. We generate CRISPRi sgRNA vectors with the best quality and best price, and our unique technology ensures fast turnaround time. If you have any questions or requirements, don't hesitate to contact us.